High – resolution melting analysis and its application for mechanobiological analysis

Maruša Lokar, Andrej Kos

Abstract


High resolution melting (HRM) is a mutation scanning technique that monitors the progressive change in fluorescence caused by the release of an intercalating DNA dye from a DNA duplex as it is denatured by marginal increases in temperature. It is a method requiring the inclusion of a saturating intercalating dye in the PCR mix and the addition of a high resolution melt step after PCR. The aim of the paper is to introduce basics of HRMA as a multipurpose technology that may be used in modified gene expression study in mechanobiology.

Keywords


HRM; mechanobiology; gene mutation.

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References


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ISSN 1801-1217 (Print)
ISSN 1805-9422 (Online)
Published by the Czech Technical University in Prague, Faculty of Mechanical Engineering